Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. The authors declare that they have no competing interests. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Search ; Wood, P.J. GYA primarily conducted research. Molecular dynamics simulations on aqueous Exp Cell Res. Careers. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Hindered diffusion of dextran and ficoll in microporous membranes. Anal. Advances in Cosmetic Surgery. Advances in chemistry series Washington, DC: American Chemical Society., pp. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. The parameters of Mark-Houwink for polymers can vary with the polymer solution and temperature. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Hi Alan F Rawle sir, Can you help me in calculating the size of dextran 6 (molecular weight= 6000) and dextran 70 (molecular weight 70000) A single frequency laser is directed to the sample contained in a cuvette. Journal of Controlled Release, 119, 5-24. Epub 2021 Feb 9. The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. Polymers | Free Full-Text | Confinement-Induced Fractionation and (2000). High Manufacturing Standards of Molecular Probes Dextrans. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Google Scholar. (1969). Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Polysaccharides-based nanoparticles as drug delivery systems. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Drug deliv. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Nanoparticles in magnetic resonance imaging: from simple to dual Correspondence to The unfolding of iRFP713 in a crowded milieu - PeerJ The basic setup of a DLS instrument is shown in Figure 1. Learn More. Would you like email updates of new search results? 151-166. Nair SS. 2012;3(1):208. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Pathan IB, Setty CM. J Pharm Sci. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Structure Properties of Dextran. European Polymer Journal, 36, 2143-2146. The hydrodynamic radius of 2-NBDG glucose was too small to measure. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Theory, Estimation, Experiment, and Data. American Journal of Food Science and Technology 2013, 1(3), 60-66. 2006;3(3):37385. Molecular weight (M v) and hydrodynamic properties can be calculated from there. Dextran 670 GPC Standard | Pharmacosmos Dextran For Research Use Only. We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Quantitative data are presented as the meanstandard deviation, and comparisons were carried out using one-way ANOVA (Systat Software Inc., Chicago, IL, USA). Epub 2022 Feb 2. Experimental and numerical study on the mixed lubrication WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. dextran Determination of intrinsic viscosities of macromolecules and nanoparticles. (2008). The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Dependence on Concentration. Macromolecules. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. For a rigid or rod like polymer molecule that is greatly extended in solution, the Mark-Houwink a constant approaches to a value of 2.0 [48].The hydrodynamic radius (RH), for a sphere ((a/b) = 2.5) is given by the Einstein relation [49]. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Carbohydrate Polymers, 36, 319-326. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. ; De Jong, S.J. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Huggins equation [41] gives the relation to the relative viscosity increase (i). With FITC-dextran 20 K, cumulative An official website of the United States government. Nanoparticles in magnetic resonance imaging: from simple to dual What is hydrodynamic radius 2009;301(8):60913. At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Figure5 shows the cumulative amount of the FITC-dextran (M.W. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. B 113, 8020-8025. PubMedGoogle Scholar. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Mark-Houwink-Kuhn-Sakurada Exponent at the Condition. Crini, G. (2005). We observe that this maximum radius is below In vivo iontophoretic delivery of salmon calcitonin across microporated skin. 10K, and c M.W. ; Weisz, J. Porcine wound models for skin substitution and burn treatment. The micropores of the human cadaver skin were regenerated according to the time. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Epub 2012 Oct 18. By using this website, you agree to our 2012;27(3):20212. Progress in Biophysical Molecules Biological, 68, 207-262. The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. Guner, Ali (1999). ; Fran, H.P. (1937). This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. WebTry out PMC Labs and tell us what you think. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. volume24, Articlenumber:22 (2020) The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. (2007). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. The effect of branching is best recognized from the molecular weight dependencies of []. Teixeira CG, Fusieger A, Milio GL, Martins E, Drider D, Nero LA, de Carvalho AF. Journal of Chemical Education, 49(8), 577-579. There is a deviation from linearity after 580kDa [69, 70]. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! dextran Ahn, G.Y., Eo, HS., Kim, D. et al. Viet, D.; Beck-Candanedo, S.; Gray D. G. (2008). Curley SA. (2001). Hydrodynamic Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Biomater Res 24, 22 (2020). This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. All authors have consented to the submission of this manuscript for publication. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). ; Aberle, T; Burchard, W. (2000). Biomaterials. Transdermal drug delivery: overcoming the skins barrier function. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Int J Food Sci Nutr. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. Analytical Chemistry. ; van Nostrum, C.F. 2005;22(4):5505. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. CAS WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Learn More. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Molecular Weight Distributions of Water-Soluble Polymers by Flow Field-Flow Fractionation. The human and pig skin permeability of FITC-dextran (M.W. 2009;6(4):34354. Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). Viswanath, D.S. Molecular weight determination of polysaccharides. Bahary, W.S. statement and Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. Journal of American Chemical Society, 64(11), 2716-2718. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Citation-(RIS Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with 2013, Article ID 360239, pp. 2016;47:15964. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Synthesis and characterization of blue dextrans The hydrodynamic radius R h, defined as the radius of a hard sphere that diffuses at the same rate as the solute, takes these effects into account. The M-H equation is empirical and valid for monodisperse polymers, and it is applied to biomacromolecules (complex, hyperbranched, crosslink), taking the precaution of comparing the value of Mv with the polydispersity index (qM-H). In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. eCollection 2018 Feb. Molecules. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Ibrahim O, Munavalli GS, Dover JS. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. However, in vitro investigations are very few. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. Radiofrequency ablation of malignant liver tumors. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. 2018;1:10915. Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. Ind. Article Springer Editions. dextran (1954). Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Ortega, A.; Garca de la Torre, J. Google Scholar. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Amersham Biosciences, AA Edition, www.amershambiosciences.com. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Recent Pat. 20K. * shows a significant difference between the M.W. Die Makromolekulare Chemie, 148(1), 229-238. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Carbohydrate Polymers 72, 356-360. (2007). Bethesda, MD 20894, Web Policies Cell permeabilization using a non-thermal plasma - IOPscience The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. After 24h, the cumulative permeation of FITC-dextran M.W. Structural and biocompatibility properties of dextran from